május 18. 08:00 - június 27. 08:00
június 29. 09:00 - július 2. 18:00
július 6. 09:00 - 10. 18:00
Microbial Alterations in Biopsy Samples of Patients with Oral Potentially Malignant Disorders
Received: 8 June 2018/Accepted: 19 July 2018 # Arányi Lajos Foundation 2018
Oral carcinogenesis often leads to the alteration of the microbiota at the site of the tumor, but data are scarce regarding the microbial communities of oral potentially malignant disorders (OPMDs). Punch biopsies were taken from healthy and nonhealthy mucosa of OPMD patients to analyze the microbiome using metagenomesequencing.
Inhealthy oralmucosabiopsiesthe bacterial phyla Firmicutes, Fusobacteria, Proteobacteria, Actinobacteria and Bacteroidetes were detected by Ion Torrent sequencing. The same phyla as well as the phyla Fibrobacteres and Spirochaetes were present in the OPMD biopsies. On the species level, there were 10 bacterial species unique to the healthy tissue and 35 species unique to the OPMD lesions where as eight species were detected in both samples. We observed that the relative abundance of Streptococcus mitis decreased in the OPMD lesions compared to the uninvolved tissue. In contrast, the relative abundance of Fusobacterium nucleatum, implicatedin carcinogenesis, waselevatedin OPMD. We detected markedly increased bacterial diversity in the OPMD lesions compared to the healthy oral mucosa. The ratio of S. mitis and F. nucleatum are characteristically altered in the OPMD lesions compared to the healthy mucosa.